BioTech Encyclopedia

Synthetic gene circuits

Experiments: basic research on living systems, not technology.

Also: synthetic biology, genetic toggle switch, repressilator, cell computing

In 2000, two landmark papers showed that genes could be wired together like electronic parts, giving bacteria a memory switch and a ticking clock. They are often seen as the start of synthetic biology.

Known properties: Synthetic gene circuits

Only what the cited papers report.

  • The repressilator used green fluorescent protein as a readout of its state in individual cells. doi:10.1038/35002125

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Timeline

  1. 2000 The genetic toggle switchThe repressilator, a genetic clock

Readiness: lab lab studies · prototype demonstrated objects · commercial products or buildings in real use · review summaries of other studies.
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lab biology 2000

The genetic toggle switch

Gardner, Cantor and Collins built a synthetic two-state (bistable) gene network in E. coli that flips between states and stays there, and gave a simple theory predicting when this works.

Built / tested: genetic toggle switch

As reported in: Gardner, Cantor, Collins (2000). Construction of a genetic toggle switch in Escherichia coli. Nature. doi:10.1038/35002131

Abstract only

lab biology 2000

The repressilator, a genetic clock

Elowitz and Leibler wired three repressor genes not from any natural clock into a loop in E. coli, making cells periodically switch green fluorescent protein on and off.

Built / tested: repressilator

As reported in: Elowitz, Leibler (2000). A synthetic oscillatory network of transcriptional regulators. Nature. doi:10.1038/35002125

Abstract only

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