BioTech Encyclopedia

Nanopore sequencing: uses in research

Also: MinION, Oxford Nanopore

Reading DNA by pulling it through a tiny pore and measuring the electrical current.

Known properties: Nanopore sequencing

Only what the cited papers report.

Uses proposed by researchers for this topic are listed on Potential uses.

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Timeline

  1. 1996 DNA through a membrane channel
  2. 2016 The MinION

Readiness: lab lab studies · prototype demonstrated objects · commercial products or buildings in real use · review summaries of other studies.
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Start here · reviewreview genomics 2016

The MinION

Jain and colleagues summarised the portable Oxford Nanopore MinION, with reads exceeding 150 kilobases and in-field pathogen detection.

Publisher update notice: correction

As reported in: Jain, Olsen, Paten et al. (2016). The Oxford Nanopore MinION: delivery of nanopore sequencing to the genomics community. Genome Biology. doi:10.1186/s13059-016-1103-0

Read free · publisher open access

genomics

lab genomics 1996

DNA through a membrane channel

Kasianowicz and colleagues showed an electric field drives single strands of RNA and DNA through a 2.6 nm channel, with blockade duration proportional to length.

As reported in: Kasianowicz, Brandin, Branton et al. (1996). Characterization of individual polynucleotide molecules using a membrane channel. Proceedings of the National Academy of Sciences. doi:10.1073/pnas.93.24.13770

Read free · preprint / author manuscript

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